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Compound: gamma-Aminobutyric Acid (GABA)
Separation of Isomers of Aminobutyric Acids |
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Application Notes: Primesep C separates the isomers of aminobutyric acids by a combination of reversed-phase and ionic interaction mechanisms. alpha-Aminobutyric acid, beta-Aminobutryic acid, and gamma-Aminobutyric acid (GABA) are baseline resolved without ion-pair reagents. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) ammonium acetate with evaporative light scattering detection (ELSD). Application Analytes: Application Detection: |
HPLC Separation of Polar Compounds |
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HPLC Separation of Amino Acids |
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Application Notes: Amino acids are building blocks for peptides and proteins. Serine is not essential to human diet and it is synthesized in human body. Methionine is not synthesized in human body and needs to be ingested. GABA is used to enhance growth of specified plants, prevent development of powdery mildew on grapes, and suppress certain other plant diseases. In humans GABA helps to maintain normal brain function. Serine, methylserine, GABA and methionine are separated on Primesep 100 column by mixed-mode mechanism. Amino acids are retained by combination of reverse phase and cation-exchange mechanisms. At lower pH carboxylic acid fragment of amino acid is suppressed and not ionized, making amino acids more basic and slightly more hydrophobic. Amount of acetonitrile, buffer concentration and buffer pH can be used to adjust retention time. Underivatized amino acids are well retained and separated with perfect peak shape and symmetry. Fast method can be used for UV, ESLD and LC/MS quantitation of serine, methylserine, GABA and methionine. Application Analytes: Application Detection: |
HPLC Separation of Serine, a-Methylserine, GABA, and Methionine Mixture |
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HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N |
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HPLC Separation of GABA and GLU |
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Application Notes: Amino acids are building blocks for peptides and proteins; they are used in various supplement formulations and as starting reagents in chemistry to introduce chirality. GABA and GLU are used to enhance growth of specified plants, prevent development of powdery mildew on grapes, and suppress certain other plant diseases. L-Glutamic acid is one of the major amino acids naturally found in plant and animal proteins, and GABA helps to maintain normal brain function. Both amino acids behave as neurotransmitters. All amino acids have both basic and acidic groups. Depending on pH amino acids can be basic, acidic or zwitter-ionic. Due to the polar nature of underivatized amino acids, analysis is a very challenging task. Derivatization and ion-pairing reagents are used to provide retention of amino acids. Simple method is developed on Primesep 100 column using combination of acetonitrile/water with phosphoric acid as a mobile phase. Method uses UV detection. Amino acids are well retained without use of ion-pairing reagents. Fast reliable method can be developed for all underivatized natural and synthetic amino acids. Application Analytes: Application Detection: |
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Copyright © SIELC Technologies. 2002-2007
Copyright © SIELC Technologies. 2002-2007