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Compound: Ascorbic Acid
Separation of Two Vitamins: Different Polarity - Isocratic Methods |
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Application Notes: Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm. Application Analytes: Application Detection: |
HPLC Method for Separation of Amino Sugar and Related Impurities on Primesep 100 HPLC Column |
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Application Notes: Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column. Application Analytes: Application Detection: |
HPLC Separation of Ascorbic and Dehydroascorbic Acids |
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HPLC Analysis of Active Drug in a Formulation |
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HPLC Analysis of ACE Serum Active Ingredients |
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HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities |
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Application Notes: Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties. Application Analytes:
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SIELC Technologies | 65 East Palatine Road, Suite 221, Prospect Heights, IL USA 60070 | Tel: (847) 229-2629 Fax: (847) 655-6079 | Email: mail@sielc.com | sitemap
Copyright © SIELC Technologies. 2002-2007
Copyright © SIELC Technologies. 2002-2007