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HPLC Separation of Human and Synthetic Insulins on Promix Column |
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Application Notes:
Application Columns:
- Promix™ MP is a mixed-mode HPLC column with general hydrophobic and ion-exclusion properties for the separation of medium peptides and proteins from 1 to 10 kDa and a broad range of PI values. The Promix MP stationary phase combines a hydrophobic chain and a basic functional group for ion exclusion of positively charged molecules. Methods can be developed on Promix columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems. Promix MP uses mobile phases of at least 1mM buffer concentration and a pH range from 2 to 5.5. Promix MP is available with 300Å and 800Å pore sizes.
Application Analytes:
Application Detection:
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