Compound: Methylmalonic Acid

HPLC Method for Separation of Amino Sugar and Related Impurities on Primesep 100 HPLC Column

Application Notes:

Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.

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Application Detection:

HPLC Separation of Succinic and Methylmalonic Acids

Application Notes:

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Application Detection:

HILIC Separation of Carboxylic Acids

Application Notes:

Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.

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