Compound: Decaffeinated Coffee

HPLC Separation of Coffee

Application Notes:

The amount of caffeine in regular and decaffeinated coffee can be determined using a Primesep SB column. The HPLC method combines reversed-phase and polar interactions to elute caffeine without interference from the coffee complex mixture. This method can be made even more robust by incorporating a diverter valve and a guard column to prevent late eluting components from sticking to the Primesep SB column. The sample is injected onto the guard column and after a defined time point, the eluent flow is reversed to elute the caffeine peak onto the analytical column without the late eluters that can shorten column life. The HPLC separation uses a mobile phase of water, methanol (MeOH) and phosphoric acid (H3PO4) and UV detection at 270 nm.

Application Columns:

Primesep^® SB is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep SB stationary phase combines a hydrophobic chain and a strongly basic functional group as an anion exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep SB columns. Primesep SB is ideal to retain hydrophobic compounds by reversed-phase retention due to its C18 carbon chain. Basic hydrophobic compounds are usually retained less on Primesep SB columns than on similar RP columns due to a charge repulsion effect. Primesep SB also separates bases by an ion-exclusion mechanism. Primesep SB demonstrates significant improvement in retention and selectivity for the separation of weak acid analytes by a combination of reversed phase and anion exchange. In this case the basic functional group acts as an embedded ion-pairing reagent. Primesep SB column is one of the best separation media for natural product extracts. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.

Application Analytes:

Application Detection:

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