Compound: Phosphate

HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC

Application Notes:

Sulfate and phosphate are separated on Primesep B2 and Primesep D column by anion-exchange mechanism. Because both sulfate and phosphate ions are not UV-active, ELSD is used to monitor separation of both anions. Method can be used to quantitate phosphate and sulfate in various pharmaceutical and chemical formulation, products and solution, drinking and ground water. Retention time is adjusted by increase or decrease of buffer concentrations. Two anions can be retained and separated on a very short column due to strong ion-exchange interaction. Method shows good reproducibility and versatility.

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HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns

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HPLC Separation of Citric Acid and Phosphate Ions in HILIC Chromatography

Application Notes:

Citric acid and phosphate ion are separated by HILIC on Primesep N HILIC HPLC column. Both compounds are very hydrophilic. Citric acid and phosphate ions are well separated with excellent peak shape. Method can be used for quantitation of citrate and phosphate in various formulations. Because of the lack of UV activity, separation can be monitored by LC/MS, ELSD or Corona CAD. Method can be used for other hydrophilic organic and inorganic acids.

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