CAS Number | 3371-50-4 |
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Molecular Formula | C18H32O16 |
Molecular Weight | 504.438 |
InChI Key | FBJQEBRMDXPWNX-FYHZSNTMSA-N |
LogP | -6.9 |
Synonyms |
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Applications:
Separation of Trisaccharides on SIELC Columns
July 6, 2015
Trisaccharides can be difficult to separate using conventional HPLC columns. The four trisaccharides panose, raffinose, isomaltotriose, and kestose have the same chemical formula, and the all have two glycosidic bonds connecting the three monosaccharides. Obelisc N was used as a stationary phase to separate trisaccharides because it is capable of multiple modes of separation. Obelisc N is a highly polar column that retains polar and charged analytes. Even though the trisaccharides differ only in regio- and stereochemistry they are resolved.
Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN – 75% |
Buffer | AmFm pH 3.0- 10 mM |
Flow Rate | 1.0 ml/min |
Detection | ELCD |
Class of Compounds |
Trisaccharides, Hydrophilic, Ionizable |
Analyzing Compounds | Raffinose, Kestose, Panose, Isomaltose |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsObelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsKestose
Panose
Raffinose