Kestose

kestose

CAS Number562-68-5
Molecular FormulaC18H32O16
Molecular Weight504.438
InChI KeyODEHMIGXGLNAKK-UHFFFAOYSA-N
LogP-5.5
Synonyms

    Applications:

    Separation of Trisaccharides on SIELC Columns

    July 6, 2015

     

    Trisaccharides can be difficult to separate using conventional HPLC columns. The four trisaccharides panose, raffinose, isomaltotriose, and kestose have the same chemical formula, and the all have two glycosidic bonds connecting the three monosaccharides. Obelisc N was used as a stationary phase to separate trisaccharides because it is capable of multiple modes of separation. Obelisc N is a highly polar column that retains polar and charged analytes. Even though the trisaccharides differ only in regio- and stereochemistry they are resolved.

    Condition 

    Column Obelisc R, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN – 75%
    Buffer AmFm pH 3.0- 10 mM
    Flow Rate 1.0 ml/min
    Detection ELCD

    Description

    Class of Compounds
    Trisaccharides,  Hydrophilic, Ionizable
    Analyzing Compounds Raffinose, Kestose, Panose, Isomaltose

     

    Application Column

    Obelisc N

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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    Obelisc R

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Isomaltotriose
    Kestose
    Panose
    Raffinose

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.