HPLC Method for Analysis of Selenious Acid on Newcrom BH Column


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Selenious Acid is used in protecting and changing the color of steel, most often in weaponry. It is highly toxic in excessive quantities, and if ingested, it could lead to death. The Acid is also capable of penetrating the skin and can produce acute poisonings. Newcrom BH, a reverse-phase column, contains embedded basic ionizable groups and can retain Selenious Acid. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Condition

Column Newcrom BH, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.1%
Flow Rate 0.5 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
 Acid, Hydrophilic, Ionizable
Analyzing Compounds Selenious Acid

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Selenious Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.