Inosine

Inosine structural formula

CAS Number58-63-9
Molecular FormulaC10H12N4O5
Molecular Weight268.230
InChI KeyUGQMRVRMYYASKQ-KQYNXXCUSA-N
LogP-2.10
Synonyms
  • Inosine
  • 9-beta-D-Ribofuranosyl-9H-purin-6-ol
  • 9H-Purin-6-ol, 9-beta-D-ribofuranosyl-
  • 58-63-9
  • 1,9-Dihydro-9-β-D-ribofuranosyl-6H-purin-6-one
  • 6H-Purin-6-one, 1,9-dihydro-9-β-D-ribofuranosyl-
  • 9-β-D-Ribofuranosylhypoxanthine
  • Hypoxanthine 9-β-D-ribofuranoside
  • Hypoxanthine ribonucleoside
  • Hypoxanthine riboside
  • Hypoxanthine, 9-β-D-ribofuranosyl-
  • Hypoxanthosine
  • inosina
  • NSC 20262
  • Oxiamin
  • Panholic-L
  • Ribonosine
  • Trophicardyl
  • EINECS 200-390-4
  • Hypoxanthine, 9-beta-D-ribofuranosyl-
  • beta-D-Ribofuranoside, hypoxanthine-9
  • Inosinum
  • UNII-5A614L51CT
  • (-)-Inosine
  • 1,9-Dihydro-9-b-D-ribofuranosyl-6H-Purin-6-one
  • 1,9-Dihydro-9-beta-D-ribofuranosyl-6H-purin-6-one
  • 1,9-Dihydro-9-beta-delta-ribofuranosyl-6H-purin-6-one
  • 9-[(2R,3R,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-3H-purin-6-one
  • 9-[(2R,3R,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]-3H-purin-6-one
  • 9-[(2R,3R,4R,5R)-3,4-dihydroxy-5-methylol-tetrahydrofuran-2-yl]-3H-purin-6-one
  • 9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6,9-dihydro-1H-purin-6-one
  • 9-b-D-Ribofuranosyl-Hypoxanthine
  • 9-b-D-Ribofuranosylhypoxanthine
  • 9-beta-D-Ribofuranosyl-Hypoxanthine
  • 9-beta-D-ribofuranosyl-9H-purin-6-ol
  • 9-beta-D-ribofuranosylhypoxanthine
  • 9-beta-delta-Ribofuranosyl-Hypoxanthine
  • 9-beta-delta-Ribofuranosylhypoxanthine
  • 9beta-D-Ribofuranosylhypoxanthine
  • 9beta-delta-Ribofuranosylhypoxanthine
  • Atorel
  • HXR
  • Hypoxanthine 9-beta-D-ribofuranoside
  • Hypoxanthine 9-beta-delta-ribofuranoside
  • Hypoxanthine nucleoside
  • Hypoxanthine-9 beta-D-Ribofuranoside
  • Hypoxanthine-9 beta-delta-Ribofuranoside
  • Hypoxanthine-9-D-ribofuranoside
  • Hypoxanthine-9-beta-D-ribofuranoside
  • Hypoxanthine-9-beta-delta-ribofuranoside
  • Hypoxanthine-9-delta-ribofuranoside
  • Hypoxanthine-ribose
  • Indole-3-carboxaldehyde
  • Ino
  • Inosie
  • Inosin
  • Iso-prinosine
  • Pantholic-L
  • Selfer
  • beta-D-Ribofuranoside hypoxanthine-9
  • beta-Inosine
  • beta-delta-Ribofuranoside hypoxanthine-9
  • hypoxanthine D-riboside
  • i
  • 12712-98-0
  • 132953-54-9
  • 28861-88-3
  • 292853-81-7
  • 4181-51-5
  • 691344-25-9
  • 740029-83-8

Applications:

HPLC Separation of Acedoben and Inosine on SHARC 1 Column

December 21, 2020


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Inosine pranobex is an antiviral drug. A combination of inosine and dimepranol acedoben (a salt of acetamidobenzoic acid and dimethylaminoisopropanol) has no effect on viral particles itself. Instead, it acts as an immunostimulant. It is most commonly used to treat the rare measles complication subacute sclerosing panencephalitis in conjunction with intrathecal interferon therapy.
Chromatography of these two compounds can be difficult due to their high polarity. But both compounds can be well retained and separated using anhydrous (water-free) conditions using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase with volatile buffer containing Formic Acid 0.1% and AmFm – 0.01%, making the method MS-compatible. Both compounds can also be UV detected at 270 nm.
 

Condition

Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer Formic Acid 0.1% , AmFm – 0.01%
Flow Rate 1.0 ml/min
Detection UV 270 nm

 

Description

Class of Compounds
Nucleoside monophosphate, Hydrophilic, Ionizable
Analyzing Compounds Inosine, Acedoben

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
4-Aminobenzoic Acid
Inosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Inosine

August 6, 2015

 

 

 

Inosine is a nucleoside made of a hypoxanthine and a ribose ring. As a drug, Inosine is useful for the treatment of various autoimmune diseases. Legacy L1 was used to retain Inosine by reverse phase mechanism. Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

Condition

Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeOH – 10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 248 nm

 

Description

Class of Compounds
 Nucleoside,  Hydrophilic, Ionizable
Analyzing Compounds Inosine

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
Inosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Inosine and Deoxyinosine

July 11, 2012

Condition

Column Sharc 1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH – 97/3%
Buffer Formic Acid 0.1% , AmFm – 0.01%
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Nucleoside monophosphate, Hydrophilic, Ionizable
Analyzing Compounds Inosine, Deoxyinosine

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
2-Deoxyinosine
Inosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.