| CAS Number | 123-76-2 |
|---|---|
| Molecular Formula | C5H8O3 |
| Molecular Weight | 116.11 |
| InChI Key | JOOXCMJARBKPKM-UHFFFAOYSA-N |
| LogP | -0.5 |
| Synonyms |
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Applications:
HPLC Separation of Levulinic acid and Sorbic Acid on Newcrom BH Column
June 30, 2020
HPLC Method for Levulinic acid, Sorbic acid on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Levulinic acid, Sorbic acid.
Levulinic acid is used as a precursor for a wide variety of compounds and is used extensively in pharmaceuticals and cosmetics industries. Despite that, when ingested, it can be harmful. It has the chemical formula C5H8O3.
Sorbic acid is used as a preservative in food, drinks, and cosmetics. It works through inhibiting the growth of molds, yeasts, and some bacteria. While it is considered safe, studies have connected it to hyperactivity in children when mixed with other additives. It has the chemical formula C6H8O2.
The two acids can be separated in HPLC using Newcrom BH mixed-mode column using a mobile phase of acetonitrile (ACN) and water and either phosphoric acid (H3PO4) or formic acid (FA) as buffer allowing the the use of either UV or MS detector.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O |
| Buffer | H3PO4, Formic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210, 266 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Levulinic acid, Sorbic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Sorbic acid
