Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Lidocaine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Lidocaine, also known as xylocaine and lignocaine, is an anesthetic and cardiac depressant used as an antiarrhythmic agent. It numbs tissue in a specific area by blocking nerve signals in the body. The medication is used to reduce pain caused by skin irritations. An overdose of numbing medicine can cause fatal side effects Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Lidocaine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.