| CAS Number | 82419-36-1 |
|---|---|
| Molecular Formula | C18H20FN3O4 |
| Molecular Weight | 361.373 |
| InChI Key | GSDSWSVVBLHKDQ-UHFFFAOYSA-N |
| LogP | -0.390 |
| Synonyms |
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Applications:
HPLC Method of Ofloxacin in Ofloxacin 0.3 % Ear Drops on Primesep D Column
October 5, 2021
HPLC Method for Ofloxacin on Primesep D by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ofloxacin
Ofloxacin is a common antibiotic with the chemical formula C18H20FN3O4. It is typically taken by mouth or injected into a vein to treat variety of bacterial infections. Those infections include pneumonia, prostatitis, and the plague. As eye and ear drops, it is also used to treat superficial bacterial infections of the eye and otitis media. As a fluoroquinolone, it is well tolerated, but may cause nausea, vomiting, and diarrhea as a side effect.
Ofloxacin can be measured at low UV. Using a Primesep D reverse-phase column and a mobile phase consisting of water and an acetonitrile (MeCN) gradient with a sulfuric acid (H2SO4) buffer, Ofloxacin can be retained. This analysis method can be UV detected at 253 nm.
| Column | Primesep D, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 10-20%, 10 min |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 253 nm |
| Class of Compounds |
Antibiotics, Drug |
| Analyzing Compounds | Ofloxacin |
Application Column
Primesep D
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Analysis of Ofloxacin
May 3, 2016
Ofloxacin is a synthetic second generation fluoroquinolone antibacterial agent that was previously used for treatment of bacterial infections. It was later replaced by less toxic medications, because Ofloxacin is harmful if swallowed and when heated to decomposition it emits toxic fumes. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Ofloxacin. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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