Ofloxacin

Ofloxacin

CAS Number82419-36-1
Molecular FormulaC18H20FN3O4
Molecular Weight361.373
InChI KeyGSDSWSVVBLHKDQ-UHFFFAOYSA-N
LogP-0.390
Synonyms
  • Ofloxacin
  • 9-Fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid
  • 7H-1,4-Oxazino[2,3,4-ij]quinoline-6-carboxylic acid, 9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-
  • 82419-36-1
  • 7H-Pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid, 9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-
  • (.+-.)-Ofloxacin
  • 7H-Pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid, 9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-, (.+-.)-
  • 9-Fluoro-2,3-dihydro-3-methyl-10-(4-methylpiperazinyl)-7-oxo-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid
  • 9-Fluoro-2,3-dihydro-3-methyl-10-(N-methylpiperazinyl)-7-oxo-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid
  • 9-Fluoro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-2,3-dihydro-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid
  • Flobacin
  • Ocuflox
  • Oflocet
  • Oflocin
  • Ofloren
  • Oflovir
  • Ofloxacine
  • Ofloxin
  • Oxaldin
  • Prifloxcin
  • Tariferid
  • Tarivid
  • Visiren
  • Zanocin
  • BRN 3657947
  • (+-)-9-Fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-7H-pyrido(1,2,3-de)(1,4)benzoxazin-6-carbonsaeure
  • Ofloxacin Otic
  • Floxin in dextrose 5%
  • Floxin in dextrose 5% in plastic container
  • Floxin Otic
  • Ofloxacina
  • Ofloxacino
  • Ofloxacinum
  • UNII-A4P49JAZ9H
  • OFLX
  • 83380-47-6

Applications:

HPLC Method of Ofloxacin in Ofloxacin 0.3 % Ear Drops on Primesep D Column

October 5, 2021

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Ofloxacin

Ofloxacin is a common antibiotic most-often used to topically treat eye and ear infections via drops. Ofloxacin can be measured at low UV. Using a Primesep D reverse-phase column and a mobile phase consisting of water and an acetonitrile (MeCN) gradient with a sulfuric acid (H2SO4) buffer, Ofloxacin can be retained. This analysis method can be UV detected at 253 nm.

 

Condition 1

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-20%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 mL/min
Detection UV 253 nm

 

Description

Class of Compounds
Antibiotics, Drug
Analyzing Compounds Ofloxacin

 

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ofloxacin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Ofloxacin

May 3, 2016

Chr_1057




Ofloxacin is a synthetic second generation fluoroquinolone antibacterial agent that was previously used for treatment of bacterial infections. It was later replaced by less toxic medications, because Ofloxacin is harmful if swallowed and when heated to decomposition it emits toxic fumes. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Ofloxacin. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ofloxacin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.