Oxymetazoline hydrochloride

Oxymetazoline hydrochloride structural formula

CAS Number2315-02-8
Molecular FormulaC16H25ClN2O
Molecular Weight296.840
InChI KeyBEEDODBODQVSIM-UHFFFAOYSA-N
LogP3.79
Synonyms
  • Oxymetazoline hydrochloride
  • 6-tert-Butyl-3-[(4,5-dihydro-1H-imidazol-2-yl)methyl]-2,4-dimethylphenol--hydrogen chloride (1/1)
  • Phenol, 3-[(4,5-dihydro-1H-imidazol-2-yl)methyl]-6-(1,1-dimethylethyl)-2,4-dimethyl-, hydrochloride (1:1)
  • 2315-02-8
  • Afrazine
  • Afrin hydrochloride
  • 6-t-Butyl-3-(2-imidazolin-2-ylmethyl)-2,4-dimethylphenol hydrochloride
  • 2,6-Dimethyl-2-(4-tertiarybutyl-3-hydroxyphenyl)methylimidazoline hydrochloride
  • Duration 12 Hour Nasal Spray
  • EINECS 219-015-0
  • Nafrine hydrochloride
  • Neonabel
  • Ocuclear
  • Oxymetazoline chloride
  • 4-Way Nasal Spray
  • Nasivin
  • Lliadine
  • LLiadin Mini Paediatric
  • Duration Nasal Spray
  • Nasivine
  • St. Joseph Nasal Spray for Children
  • Vicks Sinex
  • Visine L.R.
  • UNII-K89MJ0S5VY
  • Sudafed OM
  • Anefrin nasal
  • 2-(4-t-butyl-2,6-dimethyl-3-hydroxybenzyl)-2-imidazolinium chloride
  • oxymetazoline HCl

Applications:

HPLC Separation of EDTA, Oxymetazoline and Benzyl Alcohol in Nasal Spray

September 4, 2020

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol



Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260nm.
 


Condition
Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase Gradient MeCN – 2- 70%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm

Description
Class of Compounds Acid, Hydrophilic, Preservatives
Analyzing Compounds EDTA, Oxymetazoline, Benzyl Alcohol

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Benzyl alcohol
EDTA (Ethylenediaminetetraacetic Acid)
Oxymetazoline
Oxymetazoline hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Oxymetazoline hydrochloride on Newcrom R1 HPLC column

May 16, 2018
Separation of Oxymetazoline hydrochloride on Newcrom R1 HPLC column

Oxymetazoline hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Oxymetazoline hydrochloride
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.