| CAS Number | 335-67-1 |
|---|---|
| Molecular Formula | C8HF15O2 |
| Molecular Weight | 414.070 |
| InChI Key | SNGREZUHAYWORS-UHFFFAOYSA-N |
| LogP | 5.68 |
| Synonyms |
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Applications:
HPLC Separation of Perfluoroalkyl Acids PFBA, PFAO, PEAS on Newcrom A Column
July 21, 2020
HPLC Method for Perfluorobutyric Acid (PFBA), Perfluorooctylsulfonic acid (PFAS), Perfluorooctanoic acid (PFOA) on Newcrom A by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Perfluorobutyric Acid (PFBA), Perfluorooctylsulfonic acid (PFAS), Perfluorooctanoic acid (PFOA).
Perfluoroalkyl acids (PFAAs) such as Perfluorobutyric acid, Perfluorononanoic acid and Perfluorooctylsulfonic acid are contaminants that have increasingly been found in groundwater and drinking water systems.
Perfluoroalkyl acids (PFAAs) are also strong surfactants that have been used industrially, in the production of fluoropolymers, but they are also present as residual impurities in a wide range of polymeric consumer products e.g. used to impart water and stain repellency in textiles, papers, and boards.
We were able to separate Perfluoroalkyl acids (PFAAs) in HPLC on Newcrom A mixed-mode column using a mobile phase of acetonitrile (ACN), and water with Ammonium Formate buffer. This separation can be monitored with ELSD, CAD detection techniques.
| Column | Newcrom A, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 40% |
| Buffer | AmFm pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Perfluoroalkyl acids |
| Analyzing Compounds | Perfluorobutyric Acid (PFBA), Perfluorooctylsulfonic acid (PFAS), Perfluorooctanoic acid (PFOA) |
Application Column
Newcrom A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Perfluorooctanoic acid (PFOA)
Perfluorooctylsulfonic acid (PFAS)
HPLC Determination of Pentadecafluorooctanoic acid (PFOA) on Newcrom R1 Column
March 11, 2019
HPLC Method for Perfluorooctanoic acid (PFOA) on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Perfluorooctanoic acid (PFOA).
Pentadecafluorooctanoic acid, also known as C8 due to it’s chemical being C8HF15O2, is an industrial surfactant. It is classified by the IARC as a carcinogen to humans. Due to it not breaking down via natural processes, it is also considered a “forever chemical.” It can easily move through soil and contaminate water, leading up to it building up[ in fish and wildlife. Despite that, it has wide use across industries from upholstery, to floor wax, to fire fighting sealants. PFOA was a key point in a renowned legal case of Robert Bilott against DuPont.
Perfluorooctanoic acid (PFOA) can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H2SO4 – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds |
Acid, Hydrophobic |
| Analyzing Compounds | Perfluorooctanoic acid (PFOA) |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of PFOA on Newcrom R1 HPLC column
May 16, 2018
PFOA can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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