Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.  Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow. The dye is banned or restricted in Norway, Finland and Sweden. Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue.
Tartrazine is a yellow food coloring agent. It’s a polar trisodium salt, and like most very polar compounds it exhibits very weak to no interaction with traditional reverse phase columns. Additionally, multi-charged compounds such as Tartrazine tend to have a broad peak shape using single mode ion-exchange columns and often require large quantities of a buffer in the mobile phase due to very strong ionic interactions. By using a mixed-mode column, specifically designed in mind for multiple charges, BIST A can retain and separate dyes with perfect peak shape using a mobile phase of Acetonitrile (MeCN) and water with N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP). UV detection at 430 and 596 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Allura Red (Red #40), Sunset Yellow (Yellow 6), Tartrazine (FD&C Yellow 5), Brilliant Blue FCF (Blue 1) on BIST A Column.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Separation type: Liquid Chromatography Mixed-mode

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Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue. Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden.
Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain and separate dyes with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 400nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF, Tartrazine, Sunset Yellow FCF and Allura Red

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Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow. The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.

Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.