Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Warfarin check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

 

HPLC Method for Warfarin, Sodium warfarin on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Warfarin, Sodium warfarin Warfarin is a medication that is also known as “blood thinner”. It is used to treat and prevent blood clots. Blood clots can heighten the risk of a stroke or a heart attack which could be fatal. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain Warfarin. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Column	Newcrom R1, 3.2 x 150 mm, 3 µm, 100 A, dual ended
Mobile Phase	MeCN – 50%
Buffer	Phosphoric Acid
Flow Rate	0.5 ml/min
Detection	UV 270 nm

Class of Compounds	Medication
Analyzing Compounds	Warfarin, Sodium warfarin