| CAS Number | 332-80-9 |
|---|---|
| Molecular Formula | C7H11N3O2 |
| Molecular Weight | 169.185 g/mol |
| InChI Key | BRMWTNUJHUMWMS-LURJTMIESA-N |
| LogP | -1.50 |
| Synonyms |
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Applications:
HPLC Separation of 1-Methylhistidine and 3-Methylhistidine on Primesep 200
Application Notes: Methylhistidines are polar amino acids with very similar structures. Methylhistidines are methylated derivative of histidine found in actin. N-methylhistidine is released into urine after the breakdown of actin and myosin. Urinary output of N-methylhistidine is a reliable index of the rate of myofibrillar protein breakdown in musculature. The method for separation of 1-methylhistidine and 3-methylhistidine on Primesep 200 column was developed with LC/MS compatible conditions. This method can be used for quantitation of methylhystidines in urine and other biofluids.
Application Columns: Primesep 200
Application compounds: 1-Methylhistidine, 3-Methylhistidine
Detection technique: UV, LC/MS, ELSD/CAD
| Column | Primesep 200, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 20/80% |
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Amino acid , Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | 1-Methylhistidine, 3-Methylhistidine |
Application Analytes:
1-Methylhistidine
3-Methylhistidine