2-Amino-5-methyl-thiazole

2-Amino-5-methyl-thiazole structural formula

CAS Number7305-71-7
Molecular FormulaC4H6N2S
Molecular Weight114.170
InChI KeyGUABFMPMKJGSBQ-UHFFFAOYSA-N
LogP0.426
Synonyms
  • 2-Amino-5-methyl-thiazole
  • 5-Methyl-1,3-thiazol-2-amine
  • 2-Thiazolamine, 5-methyl-
  • 7305-71-7
  • UNII-7RAB78AF2P
  • NSC-523150
  • 2-Thiazolamine, 5-methyl-
  • 5-Methyl-2-thiazolamine
  • 5-Methyl-2-aminothiazole
  • 5-Methyl-1,3-thiazol-2-amine
  • (5-Methylthiazol-2-yl)amine
  • Meloxicam related compound B

Applications:

Separation of Model Compounds in Reversed-Phase and Mixed-Mode

April 25, 2019

Separation type: Liquid Chromatography Mixed-mode








 
Many compounds are difficult, if not impossible, to separate on reverse-phase columns in HPLC. Other compounds cannot be separated on ion-exchange columns. That’s where the mixed-mode columns come in. By using a stationary phase with both hydrophobic and ion-exchange properties, allows the chromatographer to have additional controls over separation conditions. Here, we demonstrate the separation of compounds that can’t be achieved on a C18 column. By using both an organic gradient and buffer gradient of ammonium formate (AmFm), we can separate structurally similar compounds that can’t be separated on a reverse-phase column alone.




 

Condition

Column Primesep 100, 3,2×50 mm, 2,7 µm, 100A
Mobile Phase Gradient  MeCN – 10-60%, 5 min
Buffer Gradient AmFm pH 3.5- 30 – 70 mM, 5 min
Flow Rate 1.2 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug,  Basic, Hydrophilic, Hydrophobic, Ionizable.
Analyzing Compounds Adenosine, 3,4-Difluroaniline, 4-Amino-2-chloropyridine, 5-Aminoindole, 4-Amino-3-chloropyridine, 2-Amino 5-methylthiadiazole, 4-Ethylaniline

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
2-Amino-5-Methylthiadiazole
2-Amino-5-methyl-thiazole
3,4-Difluoroaniline
4-Amino-2-Chloropyridine
4-Amino-3-Chloropyridine
4-Ethylaniline
5-Aminoindole
Adenosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of 2-Amino-5-methyl-thiazole on Newcrom R1 HPLC column

May 16, 2018
Separation of 2-Amino-5-methyl-thiazole on Newcrom R1 HPLC column

2-Amino-5-methyl-thiazole can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
2-Amino-5-methyl-thiazole
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.