2,3-Butanedione

2,3-Butanedione structural formula

CAS Number431-03-8
Molecular FormulaC4H6O2
Molecular Weight86.090
InChI KeyQSJXEFYPDANLFS-UHFFFAOYSA-N
LogP-1.34
Synonyms
  • 2,3-Butanedione
  • Butane-2,3-dione
  • 431-03-8
  • Dimethyl glyoxal
  • Diacetyl
  • 4-01-00-03644
  • 2,3-Butadione
  • 2,3-Diketobutane
  • 2,3-Dioxobutane
  • Biacetyl
  • BUTA-2,3-DIONE
  • Butandion
  • Butanedione
  • butanodiona
  • Dimethyl diketone
  • Dimethylglyoxal
  • NSC 8750
  • UN 2346
  • BRN 0605398
  • EINECS 207-069-8
  • FEMA No. 2370
  • Glyoxal, dimethyl-
  • UNII-K324J5K4HM
  • 2,3-butandione
  • Acetoacetaldehyde
  • Butadione
  • Butan-2,3-dione
  • Butane-2,3-dione
  • Butanedione [UN2346]
  • Dimethyl glyoxal
  • 151677-70-2

Applications:

HPLC Determination of 2,3-Butanedione (Diacetyl) Using 4-Nitro-o-phenylenediamine as the Derivatization Reagent

July 8, 2021

Separation type: Liquid Chromatography Reverse Phase.


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High Performance Liquid Chromatography (HPLC) Method for Diacetyl.

Diacetyl is a natural by-product of fermentation and is known to be an important flavor compound in many food products. Diacetyl is a reactive diketone in artificial butter flavors. The principal types of flavorings that use diacetyl are dairy flavors, particularly butter flavorings but also cheese, milk, and yogurt. Diacetyl is also sometimes an ingredient in the so-called brown flavors such as caramel, butterscotch, and coffee flavors. The product of the derivatization of diacetyl with NPDA can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid (H3PO4). The analysis method can be UV detected at 260 nm.

Chemicals and Reagents

  • The stock solutions of 0.1 mg/mL 2,3-Butanedione (Diacetyl) were prepared in distilled water.
  • The stock solutions of 1.0 mg/mL 4-nitro-o-phenylenediamine (NPDA) were prepared in methanol.

Derivatization Procedure

  • 0.10 mL of diacetyl stock solution,
  • 0.10 mL of HCl (0.1 M),
  • 0.60 mL of methanol, and
  • 0.20 mL of NPDA stock solution were added to a test vial.

The total solution was sonicated for 20 minutes. The resulting solution was filtered through a 0.22 μm filter membrane and injected into the chromatographic system.

Condition

Column Newcrom R1, 2.1×100 mm, 3 µm, 100A
Mobile Phase MeCN – 40%
Buffer H2SO4 – 0.2%
Flow Rate 0.2 ml/min
Detection 260 nm

Description

Class of Compounds Ketone
Analyzing Compounds 2,3-Butanedione (Diacetyl), 4-nitro-o-phenylenediamine (NPDA)

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
2,3-Butanedione
4-Nitro-o-phenylenediamine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of 2,3-Butanedione on Newcrom R1 HPLC column

February 16, 2018
Separation of 2,3-Butanedione on Newcrom C18 HPLC column

2,3-Butanedione can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
2,3-Butanedione
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.