Coenzyme q10

Coenzyme Q10

CAS Number303-98-0
Molecular Formula C59H90O4
Molecular Weight863.3
InChI KeyACTIUHUUMQJHFO-UPTCCGCDSA-N
Synonyms
  • Coenzyme Q10
  • ubidecarenone
  • 303-98-0
  • ubiquinone-10
  • CoQ10
  • Ubiquinone
  • Ubiquinone 50
  • Neuquinon
  • Justquinon
  • Emitolon
  • Heartcin
  • Inokiten
  • Terekol
  • Udekinon
  • Neuquinone
  • Ubiquinone 10
  • Coenzyme Q
  • Ubiquinone Q10
  • Luvacor
  • Vitamin Q
  • Coenzyme Q 10
  • Coenzyme Q-10
  • CoQ 10
  • Ubidecarenonum [Latin]
  • Coenzyme Q(sub 10)
  • Ubidecarenona [Spanish]
  • NSC 140865
  • CoQ(sub 10)
  • Ubiquinone Q(sub 10)
  • Q 199
  • Ube-Q
  • UNII-EJ27X76M46
  • CCRIS 9016
  • C59H90O4
  • EINECS 206-147-9
  • NSC 140665
  • CHEBI:46245
  • ACTIUHUUMQJHFO-UPTCCGCDSA-N
  • EJ27X76M46
  • NCGC00159429-02
  • Coenzyme Q10, 98%
  • DSSTox_CID_26054
  • DSSTox_RID_81308
  • DSSTox_GSID_46054
  • p-Benzoquinone, 2-(3,7,11,15,19,23,27,31,35,39-decamethyl-2,6,10,14,18,22,26,30,34,38-tetracontadecaenyl)-5,6-dimethoxy-3-methyl-
  • Ubidecarenona
  • Ubidecarenonum
  • coenzyme-Q10

Applications:

HPLC Determination of Coenzyme Q10 and Ubiquinol on Primesep D Column

February 11, 2020

HPLC Method for Coenzyme q10, Ubiquinol on Primesep D by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Coenzyme q10, Ubiquinol.

Coenzyme Q10, also known as ubiquinone, is an enzyme found in animals and is used as a dietary supplement with the chemical formula C59H90O4. It acts as a redox carrier, which means that it transfers electrons in the mitochondria. The production of ubiquinone depletes with age. Ubiquinol is a reduced form of coenzyme Q10.

The two compounds are similar in structure and can be difficult to separate in reverse-phase HPLC. Using mixed-mode Primesep D column and the mobile phase with acetonitrile (ACN) and water, without the need for buffer, CoQ10 and ubiquinol can be isocratically separated and UV detected at 275nm.

Condition
Column Primesep D
Mobile Phase MeCN/H2O – 95/5%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 275 nm, Vis 435 nm

 

Description
Class of Compounds Hydrophobic
Analyzing Compounds Coenzyme q10, Ubiquinol

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Coenzyme q10
Ubiquinol
Application Detection:
UV Detection
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.