o-coumaric acid

CAS Number614-60-8
Molecular FormulaC9H8O3
Molecular Weight164.16
InChI KeyPMOWTIHVNWZYFI-AATRIKPKSA-N
LogP1.5
Synonyms
  • 2-Hydroxycinnamic acid
  • 614-60-8
  • o-Coumaric acid
  • trans-2-Hydroxycinnamic acid
  • 2-Coumaric acid
  • trans-o-Coumaric acid
  • trans-o-Hydroxycinnamic acid
  • 2-Hydroxycinnamate
  • 2-Coumarate
  • (E)-o-Hydroxycinnamic acid
  • trans-2-Hydroxycinnamate
  • (2E)-3-(2-hydroxyphenyl)prop-2-enoic acid
  • o-Hydroxy-trans-cinnamic acid
  • 3-(2-hydroxyphenyl)acrylic acid
  • (E)-3-(2-Hydroxyphenyl)-2-propenoic acid
  • (2E)-3-(2-HYDROXYPHENYL)ACRYLIC ACID
  • 2-Hydroxycinamic acid
  • 583-17-5
  • (E)-3-(2-hydroxyphenyl)prop-2-enoic acid
  • trans-2-coumaric acid
  • (2E)-3-(2-Hydroxyphenyl)-2-propenoic acid
  • 2-Hydroxycinnamic acid, (E)-
  • CINNAMIC ACID, o-HYDROXY-, (E)-
  • UNII-23AU5FZB9C
  • (E)-2-hydroxycinnamic acid
  • CCRIS 5834
  • 2-Propenoic acid, 3-(2-hydroxyphenyl)-, (2E)-
  • trans-ortho-coumaric acid
  • EINECS 210-386-4
  • NSC 32952
  • 2-Propenoic acid, 3-(2-hydroxyphenyl)-, (E)-
  • BRN 1100900
  • 23AU5FZB9C
  • (2E)-2-hydroxycinnamic acid
  • 2-Propenoic acid, 3-(2-hydroxyphenyl)-
  • CHEMBL52564
  • 2-Hydroxycinnamic acid, predominantly trans
  • CHEBI:18125
  • 3-(2-hydroxyphenyl)prop-2-enoic acid
  • o-coumarate
  • MFCD00004379
  • (E)-3-(2-hydroxyphenyl)acrylic acid
  • 2-Hydroxy Cinnamic Acid
  • (E)-3-(2-HYDROXY-PHENYL)-ACRYLIC ACID
  • 3-(2-hydroxyphenyl)prop-2-enoate
  • cis-2-hydroxycinnamic acid
  • o-Hydroxycinnamate
  • 3-(2-Hydroxyphenyl)-2-propenoic acid
  • trans-o-Coumarate
  • (2Z)-3-(2-hydroxyphenyl)acrylic acid
  • ortho-Hydroxycinnamate
  • PubChem8214
  • (E)-Coumarinic Acid
  • (2Z)-3-(2-hydroxyphenyl)acrylate
  • trans-o-Hydroxycinnamate
  • (E)-ortho-coumaric acid
  • o-Hydroxy-trans-cinnamate
  • (E)-o-Hydroxycinnamicacid
  • bmse000347
  • Cinnamic acid, o-hydroxy-
  • WLN: QV1U1R BQ
  • SCHEMBL64885
  • QSPL 150
  • CHEBI:18176
  • trans-o-HydroxyzimtsA currencyure
  • 2-Hydroxycinnamic acid, (2E)-

Applications:

Uv-Vis Spectrum of o-Coumaric acid

February 13, 2026
Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze o-coumaric acid check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

 

 

 

 

Application Analytes:
o-coumaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Coumaric acids on Newcrom BH Column

January 27, 2021

HPLC Method for m-Coumaric acid, o-coumaric acid, p-coumaric acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of m-Coumaric acid, o-coumaric acid, p-coumaric acid.

p-Coumaric acid is a major phenolic acid with the chemical formula C9H8O3. It can be found in a wide variety of graminaceous plants.  p-Coumaric acid is a natural metabolite contained in many edible plants and its antioxidant activities in reducing oxidative stress and inflammatory reactions have been demonstrated in various experiments.

m-Coumaric acid is a hydroxycinnamic acid with the chemical formula C9H8O3. It can be found in a vinegar.  It differs from p-Coumaric acid in the position of the hydroxy substitution.

o-Coumaric acid is a hydroxycinnamic acid with the chemical formula C9H8O3. It can be found in a vinegar.  It differs from p-Coumaric acid and m-Coumaric acid in the position of the hydroxy substitution.

The three isomers of coumaric acid, m-Coumaric acid, o-coumaric acid, p-coumaric acid, can be detected at low UV.  Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, the acids can be retained and UV detected at 270nm.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4 – 1.0%
Flow Rate 1.0 ml/min
Detection 270 nm

Description
Class of Compounds Acid
Analyzing Compounds m-Coumaric acid, o-coumaric acid, p-coumaric acid

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
m-Coumaric acid
o-coumaric acid
p-coumaric acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.