| CAS Number | 118-55-8 | 
|---|---|
| Molecular Formula | C13H10O3 | 
| Molecular Weight | 214.221 | 
| InChI Key | ZQBAKBUEJOMQEX-UHFFFAOYSA-N | 
| LogP | 3.46 | 
| Synonyms | 
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Applications:
HPLC Separation of Active Compounds in Drug Formulation
January 13, 2005
An HPLC method for the separation of active drug compounds on a Primesep 200 column. The retention of compounds is achieved through reverse-phase, cation exchange and hydrophobic interactions. Benzoic acid, hyoscyamine sulfate and phenyl salicylate are baseline separated using simple mobile phases of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with a UV detector.
| Column | Primesep 200, 3.0×50 mm, 5 µm, 100A | 
| Mobile Phase | MeCN/H2O | 
| Buffer | TFA | 
| Flow Rate | 1.0 ml/min | 
| Detection | UV, 220 nm | 
| Class of Compounds | Acid, Hydrophilic, Ionizable | 
| Analyzing Compounds | Benzoic acid, Hyoscyamine Sulfate, Phenylsalicylate) | 
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsHyoscyamine Sulfate
Phenylsalicylate


