HPLC Method for Metalaxyl (UV), Propoxur, Paclobutrazol, Abamectin, Propanil, Iprodione, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metalaxyl (UV), Propoxur, Paclobutrazol, Abamectin, Propanil, Iprodione, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate.

Metalaxyl, also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate,  is a systemic fungicide with the chemical formula C₁₅H₂₁NO₄.  It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.

Propoxur is a non-systemic insecticide produced from catechol with the chemical formula C₁₁H₁₅NO₃. It has a variety of uses from turf, forestry, and agricultural pests to household pests and fleas.

Paclobutrazol (PBZ) is a synthetic triazole compound with the chemical formula C₁₅H₂₀ClN₃O. It is used to control plant growth as an antagonist of the plant hormone gibberellin. Through reducing reducing internodal growth, it causes stronger stems, greater root growth, and earlier fruitset. It can also reduce frost sensitivity in plants.

Abamectin is a insecticide with the chemical formulas  C48H72O14 and C47H70O14. It is also used to control fire ant populations. In veterinary cases, it is used to deworm horses as well as an antihelmintic.

Propanil is a contact herbicide with the chemical formula C₉H₉Cl₂NO. It is one of the most widely used herbicides in America. It works as an inhibitor of photosynthesis and CO₂ fixation in weeds.

Iprodione is a hydantoin fungicide and nematicide with the chemical formula C₁₃H₁₃Cl₂N₃O₃. It is used on a wide variety of crops that are affected by fungal diseases such as botrytis bunch rot, brown rot, and Sclerotinia.  Approval of using it is different across the world.

Dursban is a highly toxic organophosphate with the chemical formula C₉H₁₁Cl₃NO₃PS. It works through interrupting the electrochemical processes in nerves.  This leads to a build-up of acetylcholine, which leads to paralysis and eventual death.

Pentachlorophenol is a manufactured chemical with the chemical formula C₆HCl₅O. It is most often used as herbicide, insecticide, fungicide, algaecide, and disinfectant. Exposure to it can cause damage to liver, kidney, blood, lungs, eyes, skin, and mouth. It is classified as a probable human carcinogen.

Fenvalerate is synthetic pyrethroid insecticide with the chemical formula C₂₅H₂₂ClNO₃. It is used against a wide range of pests, partially due to it’s moderate mammalian toxicity following short-term and acute exposure. It is the most toxic to bees and fish.  Upon contact, it may irritate skin and eyes.

Metalaxyl (UV), Propoxur, Paclobutrazol, Abamectin, Propanil, Iprodione, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.

HPLC Method for 6-Benzylaminopurine, Metalaxyl (UV), Prochloraz, Paclobutrazol, Propanil, Chlorothalonil, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate, Abamectin, Procymidone, Bifenazate on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine, Metalaxyl (UV), Prochloraz, Paclobutrazol, Propanil, Chlorothalonil, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate, Abamectin, Procymidone, Bifenazate.

6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C₁₂H₁₁N₅. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.

Metalaxyl, also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate,  is a systemic fungicide with the chemical formula C₁₅H₂₁NO₄.  It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.

Prochloraz is an imidazole fungicide with the chemical formula C₁₅H₁₆Cl₃N₃O₂. It is used widely across Europe, Asia, South America, and Australia, but is not registered for use in the United States. It works as an inhibitor of the enzyme lanosterol 14α-demethylase.

Paclobutrazol (PBZ) is a synthetic triazole compound with the chemical formula C₁₅H₂₀ClN₃O. It is used to control plant growth as an antagonist of the plant hormone gibberellin. Through reducing reducing internodal growth, it causes stronger stems, greater root growth, and earlier fruitset. It can also reduce frost sensitivity in plants.

Procymidone is a pesticide and fungicide with the chemical formula  C₁₃H₁₁Cl₂NO₂. It is typically used to kill unwanted ferns, nettles, and fungi in lupins, grapes, stone fruit, and strawberries.

Propanil is a contact herbicide with the chemical formula C₉H₉Cl₂NO. It is one of the most widely used herbicides in America. It works as an inhibitor of photosynthesis and CO₂ fixation in weeds.

Chlorothalonil is a compound with a variety of uses as a fungicide, wood protectant, pesticide, and acaricide. It is used predominantly on peanuts, potatoes, and tomatoes. Outside of agriculture, it is also used in paints, resins, emulsions, and coatings. It’s chemical formula is C₈Cl₄N₂.

Abamectin is a insecticide with the chemical formulas  C48H72O14 and C47H70O14. It is also used to control fire ant populations. In veterinary cases, it is used to deworm horses as well as an antihelmintic.

Bifenazate is pesticide with the chemical formula C₁₇H₂₀N₂O₃. It is typically used to control a large variety of mite pests in plants in various settings. It works through modulating GABA receptors and inhibiting complex III of the mitochondrial electron transport chain.

Dursban is a highly toxic organophosphate with the chemical formula C₉H₁₁Cl₃NO₃PS. It works through interrupting the electrochemical processes in nerves.  This leads to a build-up of acetylcholine, which leads to paralysis and eventual death.

Pentachlorophenol is a manufactured chemical with the chemical formula C₆HCl₅O. It is most often used as herbicide, insecticide, fungicide, algaecide, and disinfectant. Exposure to it can cause damage to liver, kidney, blood, lungs, eyes, skin, and mouth. It is classified as a probable human carcinogen.

Fenvalerate is synthetic pyrethroid insecticide with the chemical formula C₂₅H₂₂ClNO₃. It is used against a wide range of pests, partially due to it’s moderate mammalian toxicity following short-term and acute exposure. It is the most toxic to bees and fish.  Upon contact, it may irritate skin and eyes.

6-Benzylaminopurine, Metalaxyl (UV), Prochloraz, Paclobutrazol, Propanil, Chlorothalonil, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate, Abamectin, Procymidone, Bifenazate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.

HPLC Method for Chlorpyrifos-methyl (Dursban) on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorpyrifos-methyl (Dursban).

Dursban is a highly toxic organophosphate with the chemical formula C₉H₁₁Cl₃NO₃PS. It works through interrupting the electrochemical processes in nerves.  This leads to a build-up of acetylcholine, which leads to paralysis and eventual death.

Chlorpyrifos-methyl (Dursban) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an Ammonium Formate buffer. Detection is performed using UV.

Chlorpyrifos-methyl, an organophosphate pesticide is considered a target pesticide by the EURL (European Union Reference Laboratory) for the EUPT-FF9 2015 a proficiency test for the analysis of a over a hundred pesticides. This compound was separated on two different mixed-mode columns, Primesep 100 and Obelisc R. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.