Pararosaniline Hydrochloride

Pararosaniline

CAS Number569-61-9
Molecular FormulaC19H18ClN3
Molecular Weight323.824
InChI KeyJUQPZRLQQYSMEQ-UHFFFAOYSA-N
Synonyms
  • Basic Red 9
  • Pararosaniline hydrochloride
  • Pararosaniline chloride
  • Parafuchsine
  • Parafuchsin
  • p-Fuchsin
  • Basic fuchsin
  • CI Basic Red 9
  • C.I. Basic Red 9
  • Parafuksin
  • Fuchsine SP
  • Fuchsine SPC
  • BASIC PARAFUCHSINE
  • Calcozine magenta N
  • p-Rosaniline hydrochloride
  • Fuchsine DR-001
  • C.I. 42500
  • C.I. Basic Red 9 monohydrochloride
  • Paraosaniline hydrochloride
  • 4,4'-((4-Iminocyclohexa-2,5-dien-1-ylidene)methylene)dianiline hydrochloride
  • Parafuchsin [German]
  • p-rosaniline
  • Orient Para Magenta Base
  • Schultz-tab No. 779
  • NCI-C54739
  • CI Basic Red 9, monohydrochloride
  • p-Rosaniline HCl
  • p-Rosaniline.HCl
  • Cerven zasadita 9 [Czech]

Applications:

HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column

December 7, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column
HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column by SIELC Technologies

Pararosaniline (Basic Red 9) is a popular basic magenta dye and part of the triarylmethane family of dyes. Crystal Violet (Methyl Violet 10B), another basic triarylmethane dye, is frequently used for histological stains and for identifying Gram-positive bacteria. Crystal Violet Lactone, a derivative of Crystal Violet, is a basic thermochromic dye widely used as a security marker for various types of fuels. These three basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Pararosaniline, Crystal Violet and Crystal Violet Lactone

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 80/20%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 540, 590, 200 nm
Peak Retention Time3.15, 7.24, 8,82 min

Description

Class of CompoundsDyes
Analyzing CompoundsPararosaniline, Crystal Violet and Crystal Violet Lactone

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Crystal Violet
Crystal Violet Lactone
Pararosaniline Hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 Column

December 7, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 Column
HPLC Method for Analysis of Pararosaniline and Ethyl Red on Primesep 100 Column by SIELC Technologies

Pararosaniline (Basic Red 9) is a popular basic magenta dye and part of the triarylmethane family of dyes. Ethyl Red is a similarly-colored, single-charged basic dye. These two basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the visible regime at 520 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Pararosaniline and Ethyl red

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 50/50%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 520 nm
Peak Retention Time10.82 min

Description

Class of CompoundsDyes
Analyzing CompoundsPararosaniline, Ethyl red

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ethyl red
Pararosaniline Hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Dyes

March 1, 2018

HPLC Separation of Dyes

Alizarin a red pigment that comes as a red powder with a slight orange tint. When dissolved it appears bright red with a slight pink undertone. Alizarin is also known as Mordant Red 11 or Turkey Red. Eosin Y is a pigment that comes as an orange powder that when dissolved in water turns bright orange. Eosin is a fluorescent staining agent. It is used to stain red blood cells, proteins on cytoplasm, collage, and muscle fibers. It is also fluorescence. Fluorescein is a dye that comes in a dark orange powder and is used as a tracer and an indicator. As it says in the name, it is also Fluorescent, obtaining a green-yellow color when under black light. Methyl red an indicator that turns red in acidic solutions (below 4.4 pH), is a shade of orange between 4.5 and 6.1 pH, and turns bright yellow in basic solutions (above pH 6.2). Nile blue, also known as Nile blue A, is used as a stain used in biology and histology as well as a pH indicator. In pH below 7 it becomes lighter shades of glue, and at pH 0 becomes almost completely translucent. At pH of seven it has a dark blue shade, while at pH higher than that it starts becoming more red. Pararosaniline Hydrochloride, also known as Basic Red 9, or C.I. 42500. It is a a magenta solid that is used as a dye. Patent Blue Violet, also known as Patent Blue, is a deep blue pigment that is often used to dye clothes. Resazurin is a blue dye as well as a pH indicator. At pH 3.8 or lower, it is bright orange, but the higher the pH the more blue it becomes, the color stops changing at the pH of 6.5 where it becomes indigo. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain dyes. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
3-Aminoacridine
Alizarin
Carmine
Eosin Y
Fluorescein
Fluorescein
Methyl Red
Nile Blue A
Pararosaniline Hydrochloride
Patent Blue Violet (Patent Blue)
Resazurin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.