CAS Number | 57-67-0 |
---|---|
Molecular Formula | C7H10N4O2S |
Molecular Weight | 214.25 |
InChI Key | BRBKOPJOKNSWSG-UHFFFAOYSA-N |
LogP | -0.7 |
Synonyms |
|
Applications:
HPLC Separation of Antibiotics
June 11, 2020
Antibiotics are widely used for treatment and prevention of bacterial infections. Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups. Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups. In addition, methanol can be used in the mobile phase on Newcrom A column. The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer. UV detection at 275nm.
Column | Primesep 200, 4.6×100 mm, 5 µm, 100A |
Mobile Phase | MeCN Gradient |
Buffer | H2SO4 – 0.5% |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm |
Column | Newcrom A, 3.2×100 mm, 5 µm, 100A |
Mobile Phase | MeOH Gradient |
Buffer | H2SO4 Gradient |
Flow Rate | 0.5 ml/min |
Detection | UV 275 nm |
Class of Compounds | Drugs, Antibiotics |
Analyzing Compounds | Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsNewcrom A
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsSulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline