Uridine 5′-diphosphogalactose disodium salt

CAS Number28053-08-9
Molecular FormulaC15H22N2Na2O17P2
Molecular Weight610.27
InChI KeyPKJQEQVCYGYYMM-OUJOOSCPSA-L
Synonyms
  • UDP-Glc hydrate, UDPG hydrate

Applications:

HPLC Determination of Uridine Diphosphate Galactose on Newcrom B Column

May 13, 2021

Separation type: Liquid Chromatography Mixed-mode





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High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine Diphosphate Galactose









Uridine Diphosphate Galactose (UDP-galactose) is an intermediary formed in the biosynthesis of polysaccharides.
UDP-galactose can be detected in the low UV regime. Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose can be retained and separated. This analysis method can be UV detected at 265 nm, or via charged aerosol detection (CAD), both with high resolution.




Condition

Column Newcrom B, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm – pH 3.0 – 20 mm
Flow Rate 1.0 ml/min
Detection CAD, 262 nm

 

Description

Class of Compounds
Sugar Phosphate
Analyzing Compounds Sodium, Uridine Diphospate Galactose

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Uridine 5′-diphosphogalactose disodium salt
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Uridine Sugar Diphospates on Primesep N Column

May 11, 2021


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Gadolinium-DOTA


Uridine sugar diphospates are a class of complex nucleotide sugars that are integral to metabolic processes in the cell. Uridine diphosphate galactose (UDP-Galactose), Uridine diphosphate glucose (UDP-Glucose), and uridine diphosphate N-acetylglucosamine (UDP-N-acetylgalactoseamine or UDP-GlcNAc) are some of the most well-known nucleotide sugars.
These three uridine sugar diphosphate compounds can be detected in the low UV regime. Using a Primesep N normal-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose, UDP-glucose, and UDP-GIcNAc can be retained, separated, and analyzed. This analysis method can be UV detected at 260 nm.




Condition

Column Primesep N, 4.6×250 mm, 3 µm, 100A
Mobile Phase MeCN – 80%
Buffer Ammonium formate pH 3.0 – 10 mM
Flow Rate 1.0 ml/min
Detection UV 260 nm

 

Description

Class of Compounds Sugar, Phospate
Analyzing Compounds Uridine-5′-diphosphate-glucose, Uridine 5′-diphospho-N-acetylgalactosamine disodium salt, Uridine 5′-diphosphogalactose disodium salt

 

 

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Uridine 5′-diphosphogalactose disodium salt
Uridine 5′-diphospho-N-acetylgalactosamine
Uridine-5′-diphosphate-glucose
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.