Nebivolol

CAS Number	99200-09-6
Molecular Formula	C22H25F2NO4
Molecular Weight	405.4
InChI Key	KOHIRBRYDXPAMZ-UHFFFAOYSA-N
LogP	3
Synonyms	Nebivolol 99200-09-6 2,2'-Azanediylbis(1-(6-fluorochroman-2-yl)ethanol) CHEBI:64019 Nebivololum [Latin] 118457-14-0 2,2'-iminobis[1-(6-fluoro-3,4-dihydro-2H-chromen-2-yl)ethanol] R 67145 R 065824 1-(6-fluoro-3,4-dihydro-2H-chromen-2-yl)-2-[[2-(6-fluoro-3,4-dihydro-2H-chromen-2-yl)-2-hydroxyethyl]amino]ethanol (-)-Nebivolol; Levonebivolol; l-Nebivolol 118457-16-2 R65,824 R-65824 (1R)-1-[(2S)-6-fluoro-3,4-dihydro-2H-chromen-2-yl]-2-[[(2R)-2-[(2S)-6-fluoro-3,4-dihydro-2H-chromen-2-yl]-2-hydroxyethyl]amino]ethanol R 67138;d-Nebivolol;(+)-Nebivolol; (S,R,R,R)-Nebivolol Narbivolol Nebivolol-d4

Applications:

HPLC Method for Nebivolol Analysis in Bystolic Tablet Dosage Form on Primesep SB Column

September 8, 2021

View Application

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nebivolol.

Nebivolol is used to treat high blood pressure. Nebivolol can be retained in HPLC on Primesep SB reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. UV detection at 285 nm.

Condition
Column	Primesep SB, 4.6×150 mm, 3 µm, 100A
Mobile Phase	MeCN/H2O – 25/75%
Buffer	H2SO4 – 0.1%
Flow Rate	1.0 ml/min
Detection	UV 285 nm

Description
Class of Compounds	Drug
Analyzing Compounds	Nebivolol

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Bystolic
Nebivolol

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.