Histidine methyl ester

CAS Number1499-46-3
Molecular FormulaC7H11N3O2
Molecular Weight169.18
InChI KeyBXRMEWOQUXOLDH-LURJTMIESA-N
LogP-0.6
Synonyms
  • Methyl L-histidinate
  • Histidine methyl ester
  • 1499-46-3
  • L-HISTIDINE METHYL ESTER
  • methyl (2S)-2-amino-3-(1H-imidazol-5-yl)propanoate
  • UNII-9IHZ1723UE
  • CHEMBL54664
  • 9IHZ1723UE
  • L-Histidine methyl
  • PVH
  • EINECS 216-109-3
  • L-Histidinemethyl ester
  • (S)-methyl histidinate
  • H-His-OCH3
  • (S)-Methyl 2-amino-3-(1H-imidazol-4-yl)propanoate
  • L-Histidine, methyl ester

Applications:

HPLC Method for Separation of Histidine, Histidine Methyl Ester and Histidine lauryl Ester on BIST B+ Column

March 23, 2023

HPLC Method for Separation of Histidine, Histidine Methyl Ester, and Histidine Lauryl Ester on BIST B+ by SIELC Technologies

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Separation of Histidine, Histidine Methyl Ester and Histidine lauryl Ester on BIST B+ Column by SIELC Technologies.
HPLC Method for Separation of of  Histidine, Histidine Methyl Ester and Histidine lauryl Ester on BIST B+ Column by SIELC Technologies

Histidine is a naturally occurring essential amino acid that the body uses to repair damaged tissue and generate new blood cells. Histidine Methyl Ester is a histidine decarboxylase inhibitor and can be used to synthesize other biological compounds. Histidine Lauryl Ester is another derivative of Histidine. Using SIELC’s newly introduced BIST™ method, Histidine and its derivatives can be retained and separated on a positively charged, anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged dipeptide to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Histidine and its derivatives can be UV detected at 220 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Histidine, Histidine Methyl Ester and Histidine lauryl Ester

Condition

ColumnBIST B+, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 80 – 50%, 10 min
BufferH2SO4 – 0.2%
Flow Rate0.5 ml/min
DetectionUV 220 nm
Peak Retention Time2.2 min, 7.5 min, 8.9 min

Description

Class of CompoundsAmino acid
Analyzing CompoundsHistidine methyl ester, Histidine

Application Column

BIST B+

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Histidine
Histidine methyl ester
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method For Analysis Of Histidine and Histidine Methyl Ester Dihydrochloride on Primesep 100 Column

January 27, 2022

Separation type: Liquid Chromatography Mixed-mode

 


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Histidine and Histidine Methyl Ester Dihydrochloride

Histidine and Histidine Methyl Ester Dyhydrochloride are related compounds with interesting uses. Histidine is used in biosynthesis to create proteins, while histidine methyl ester dihydrochloride (a derivative of histidine) is used to create various other compounds.
This amino acid and its derivative can be detected in the low UV regime. Using a Primesep 100 mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, Histidine and Histidine Methyl Ester Dyhydrochloride can be retained, separated, and analyzed. This analysis method can be UV detected at 200 nm.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 60/40%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Amino acid
Analyzing Compounds Histidine,  Histidine Methyl Ester

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Histidine
Histidine methyl ester
L-Histidine hydrochloride monohydrate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.