Gadolinium-DOTA

CAS Number83678-67-5
Molecular FormulaC16H24GdN4O8-
Molecular Weight557.6
InChI KeyGFSTXYOTEVLASN-UHFFFAOYSA-J
Synonyms
  • gadolinate(1-), (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetato(4-)-
  • gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate
  • gadolinium DOTA
  • gadoterate
  • gadoterate sodium
  • Gd-DOTA

Applications:

HPLC Method For Analysis Of Gadolinium-DOTA on Newcrom BH Column

February 10, 2022

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Gadolinium-DOTA

Gadolinium-DOTA, or known Gadoteric acid, is a well-known contrast agent for imaging of (often leaky) blood vessels, the brain, and spine. This compound is composed of dodecane tetraacetic acid, or DOTA, with a Gadolinium III cation in the center. Due to its unique structure, this acidic compound has paramagnetic properties; when exposed to a magnetic field, it develops a magnetic moment, which can be utilized for magnetism-based imaging like MRI and NMR. Gadolinium-DOTA acts as a signal amplifier, thus appearing brighter under MRI scans and highlighting the tissues that have absorbed it. In its salt form (with meglumine), it crosses the blood-brain barrier and allows for imaging of the brain and other areas of the nervous system. Due to its paramagnetic properties, Gadolinium-DOTA fluoresces; it excites at 274 nm and emits light at 312 nm.
This acid can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of (mostly) water and acetonitrile, Gadolinium-DOTA can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution.

 

Condition

Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Acid
Analyzing Compounds Gadolinium-DOTA

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Gadolinium-DOTA
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.