Colistin A

CAS Number7722-44-3
InChI KeyXDJYMJULXQKGMM-HHAJOKTESA-N
LogP-2.8
Synonyms
  • Colistin A
  • UNII-500HI50Z9H
  • 7722-44-3
  • CHEMBL3781027
  • Polymixin E1
  • 500HI50Z9H
  • (S)-N-((S)-4-amino-1-(((2S,3R)-1-(((S)-4-amino-1-oxo-1-(((3S,6S,9S,12S,15R,18S,21S)-6,9,18-tris(2-aminoethyl)-3-((R)-1-hydroxyethyl)-12,15-diisobutyl-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptaazacyclotricosan-21-yl)amino)butan-2-yl)amino)-3-hydroxy-1-oxobutan-2-yl)amino)-1-oxobutan-2-yl)-6-methyloctanamide
  • BRN 0604503

Applications:

HPLC Method for Analysis of Colistin A on BIST B+ Column

June 3, 2022

Separation type: Bridge Ion Separation Technology, or BIST™


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HPLC Method for Analysis of Colistin A on BIST B+ Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Colistin A

Colistin A is a last-resort antibiotic drug sued to treat Gram-negative bacterial infection, such as pneumonia. Using SIELC’s newly introduced BIST™ method, Colistin A can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Colistin A can be separated, retained, and UV detected at 200 nm

Condition

ColumnBIST™ B+, 4.6×50 mm, 5µm, 100A
Mobile PhaseMeCN – 70%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm

Description

Class of CompoundsDrugs, Antibiotic
Analyzing CompoundsColistin A

Application Column

BIST B+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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Application Analytes:
Colistin A
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.