DMSO (Dimethyl sulfoxide)

CAS Number67-68-5
Molecular FormulaC2H6OS or (CH3)2SO
Molecular Weight78.129
InChI KeyIAZDPXIOMUYVGZ-UHFFFAOYSA-N
LogP-0.6
Synonyms
  • dimethyl sulfoxide
  • DMSO
  • Methyl sulfoxide
  • 67-68-5
  • Methylsulfinylmethane
  • Dimethylsulfoxide
  • Dimethyl sulphoxide
  • Methane, sulfinylbis-
  • Demsodrox
  • Demasorb
  • Demavet
  • Dimexide
  • Domoso
  • Dromisol
  • Durasorb
  • Infiltrina
  • Somipront
  • Syntexan
  • Deltan
  • Demeso
  • Dolicur
  • Hyadur
  • sulfinylbismethane
  • Dimethyl sulfur oxide
  • Dermasorb
  • Dipirartril-tropico
  • Doligur
  • Kemsol
  • Topsym
  • Gamasol 90
  • Sclerosol
  • Sulfinylbis(methane)
  • Rimso-50
  • Dimethylsulfoxid
  • Dimethylsulfoxyde
  • Rimso 50
  • Dimethylsulphoxide
  • Caswell No. 381
  • SQ 9453
  • Dimetil sulfoxido
  • Sulfinylbis-methane
  • Dimethyli sulfoxidum
  • Topsym (rescinded)
  • Rimso-5
  • NSC-763
  • methanesulfinylmethane
  • Domoso (Veterinary)
  • CCRIS 943
  • (methylsulfinyl)methane
  • methylsulfoxide
  • (CH3)2SO
  • Dimexidum
  • DMS-90
  • sulfinyldimethane
  • NSC 763
  • Dimetilsolfossido [DCIT]
  • A 10846
  • Dimethyl sulpoxide
  • M 176
  • UNII-YOW8V9698H
  • HSDB 80
  • Methane, 1,1'-sulfinylbis-
  • EPA Pesticide Chemical Code 000177
  • DMS 70
  • DMS 90
  • DMS-70
  • Dimethylsulfoxyde [INN-French]
  • AI3-26477
  • Dimetil sulfoxido [INN-Spanish]
  • Dimethyli sulfoxidum [INN-Latin]
  • Methyl Sulfoxide: DMSO
  • EINECS 200-664-3
  • CHEMBL504
  • S(O)Me2
  • NSC763
  • YOW8V9698H
  • CHEBI:28262
  • IAZDPXIOMUYVGZ-UHFFFAOYSA-N
  • MFCD00002089
  • SQ-9453

Applications:

HPLC Separation of Mixture of DMF and DMSO

April 25, 2019


Dimethylformamide, DMF, is primarily used as an organic solvent and is miscible with water and most other organic liquids. Dimethyl sulfoxide (DMSO) is also a solvent. It can dissolve both polar and nonpolar compounds and is miscible with water and other organic liquids. Both compounds can be retained and separated using anhydrous (water-free) conditions using HPLC by SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase without the need for a buffer. UV detection used at 220nm.

Condition

Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 98-85 %, 10 min
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 220 nm

 

Description

Class of Compounds
Drug, Basic, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds DMF, DMSO

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
DMF (Dimethylformamide)
DMSO (Dimethyl sulfoxide)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography

July 16, 2009

Dimethyl sulfoxide is important polar aprotic solvent, which is frequently used in pharmaceutical drug manufacturing, desolution, etc. DMSO is used as one of the solvents on protein chemistry due to universal ability to dissolve small molecules like amino acids. Amino acids and DMSO are very polar and have no retention on reversed-phase columns. In this HPLC application DMSO and glycine are separated on Primesep 100 mixed-mode column. DMSO is retained by weak reversed-phase mechanism and very low organic concentration is required in order to achieve any retention. Glycine, like any other underivatized amino acid, is retained by weak reversed-phase and moderate cation-exchange mechanism. Method uses acetonitrile/water/TFA gradient and UV detection.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds DMSO, Glycine

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Amino Acids
DMSO (Dimethyl sulfoxide)
Glycine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.