Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 5-30%, 10 min, 5 min hold |
Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 5 min hold |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Josamycin is a macrolide antibiotic effective against gram-positive bacteria. It is highly polar and does not retain well on reversed-phase columns. The Mixed-mode column Obelisc R was used to retain josamycin and its related impurities. Primesep D was also used to analyze josamycin impurities because Primesep D does not retain high-molecular weight plasma components. This method is compatible with LC/MS, prep chromatography and can be used for analysis of josamycin in biofluids.
Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 5-50%, 10 min, 3 min hold |
Buffer | H2SO4 – 0.1% |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds |
Antibiotic, Drug, Hydrophobic, Ionizable |
Analyzing Compounds | Josamycin |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsPrimesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsUV Detection