Analysis of Antibiotic Josamycin and Related Impurities in Mixed-Mode Chromatography

 

Condition 1

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-30%, 10 min, 5 min hold
Buffer Gradient Formic Acid - 0.05%-0.3%, 10 min, 5 min hold
Flow Rate 1.0 ml/min
Detection UV, 250 nm
Josamycin is a macrolide antibiotic effective against gram-positive bacteria. It is highly polar and does not retain well on reversed-phase columns. The Mixed-mode column Obelisc R was used to retain josamycin and its related impurities.  Primesep D was also used to analyze josamycin impurities because Primesep D does not retain high-molecular weight plasma components. This method is compatible with LC/MS, prep chromatography and can be used for analysis of josamycin in biofluids.

Condition 2

Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%, 10 min, 3 min hold
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds  Josamycin
 

Application Analytes:

Josamycin