Analysis of Antibiotic Norfloxacin and Related Impurities in Mixed-Mode Chromatography

 

Condition 1

Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%, 10 min, 3 min hold
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm
  The antibiotic norfloxacin is a first generation fluoroquinolone. It is effective against both gram positive and gram negative bacteria. Analysis was done on Obelisc R and Primesep D.  

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-30%, 10 min,  5 min hold
Buffer Gradient Formic Acid - 0.05%-0.3%, 10 min,  5 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds  Norfloxacin
 

Application Analytes:

Norfloxacin