| Column | Primesep D, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 5-50%, 10 min, 3 min hold |
| Buffer | H2SO4 - 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
The antibiotic norfloxacin is a first generation fluoroquinolone. It is effective against both gram positive and gram negative bacteria. Analysis was done on Obelisc R and Primesep D.
| Column | Obelisc R, 2.1x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 5-30%, 10 min, 5 min hold |
| Buffer | Gradient Formic Acid - 0.05%-0.3%, 10 min, 5 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Norfloxacin |
Application Analytes:
Norfloxacin