Analysis of Antibiotic Tylosin and Related Impurities in Mixed-Mode Chromatography

Tylosin is a macrolide antibiotic with a broad spectrum of activity against gram-negative and gram-positive organisms. It is highly polar and basic molecule with limited retention in reversed-phase chromatography. Tylosin and related impurities were analysed on Obelisc R trimodal column. Method is compatible with LC/MS and prep chromatography and can be used for HPLC analysis of tylosin in various biofluids.

Application Analytes: