Lactic acid (milk acid) is a hydrophilic acid which plays important role in many bioprocesses. Lactic acid has a tendency to self-condense to form dimers, trimers and high molecular weight oligomers. Lactic acid and it’s oligomers are retained by a combination of weak reversed-phase and weak anion-exchange mechanisms on Primesep D mixed-mode HPLC column. Retention time is controlled by amount of acetonitrile, buffer concentration and buffer pH. Method is compatible with LC/MS and other detection techniques and can be used for analysis of lactic acid in various matrices.
|Column||Primesep D, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 30/70%|
|Buffer||AmAc pH 4.0- 20 mM|
|Flow Rate||1.0 ml/min|
|Class of Compounds||Acid, Hydrophilic, Ionizable|
|Analyzing Compounds||Lactic Acid|