Analysis of Nicotinic Acid in Nicotinamide

Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Primesep 100 was used to separate nicotinamide and nicotinic acid. Concentrations of nicotinic acid as low as 0.1ppm could be used.Primesep 100 separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer Na2HPO4  pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection UV, 230 nm


Class of Compounds  Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Nicotinic Acid, Nicotinamide

Application Analytes:

Nicotinic Acid