Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column


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Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH  was used to separate nicotinamide and nicotinic acid.  Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
 Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Nicotinic Acid, Nicotinamide, Vitamin B3

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Niacin
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Application Detection:
UV Detection
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