HPLC Analysis of Chloramphenicol

Chloramphenicol is a broad-spectrum antibiotic useful in treating conjunctivitis and many other infections. It is notable as an antibiotic for developing nations due to its low cost. It was analyzed on a Legacy L1 column which uses reverse-mode to retain hydrophobic compounds. Comparisons between Legacy L1 and Phenomenex columns are available upon request.


Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeOH - 45%
Buffer Acetic Acid - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 280 nm


Class of Compounds  Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Chloramphenicol

Application Analytes: