HPLC Ananlysis of Hydrocortisone

  Hydrocortisone is the drug form of the steroid hormone cortisol. Cortisol is an important biological molecule which affects blood sugar, metabolism and the immune system. It was retained on the reverse-phase column Legacy L1. Comparisons of Legacy L1 and Phenomenex columns are available by request.


Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN - 40%
Buffer NaH2PO4 pH 2.5 - 10 mM
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds Hormone,   Hydrophobic, Ionizable
Analyzing Compounds Hydrocortisone

Application Analytes: