Toluidine isomers and aminobyphenyl isomers are organic compounds each with a substituted amine group. Primesep 200 was used to separate these isomers. Primesep 200 separates isomers by interacting with polar molecules, retaining basic compounds, and additionally by reverse phase mechanism.
| Column | Primesep 200, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm Ph 3.5 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Hydrophobic, Ionizable |
| Analyzing Compounds | o-Toluidine, 2-Aminobiphenyl, m-Toluidine, p-Toluidine, 4-Aminobiphenyl |
Application Analytes:
2-Aminobiphenyl
4-Aminobiphenyl
m-Toluidine
o-Toluidine
p-Toluidine