Separation of Trisaccharides on SIELC Columns

  Trisaccharides can be difficult to separate using conventional HPLC columns. The four trisaccharides panose, raffinose, isomaltotriose, and kestose have the same chemical formula, and the all have two glycosidic bonds connecting the three monosaccharides. Obelisc N was used as a stationary phase to separate trisaccharides because it is capable of multiple modes of separation. Obelisc N is a highly polar column that retains polar and charged analytes. Even though the trisaccharides differ only in regio- and stereochemistry they are resolved.

Condition 

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 75%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELCD

Description

Class of Compounds Trisaccharides,  Hydrophilic, Ionizable
Analyzing Compounds Raffinose, Kestose, Panose, Isomaltose
 

Application Analytes:

Isomaltotriose
Kestose
Panose
Raffinose