2,4-D Retention on Primesep 100 and Obelisc R

  2,4-D makes up half of the formula for Agent Orange, the defoliant that was used during the Vietnam War and Malayan Emergency. Commonly used in lawn and turf pesticides, 2,4-D is typically mixed with other pesticides such as mecoprop and dicamba. It was analyzed on Primesep 100 and Obelisc R. Primesep 100 separates using reverse-phase and acidic ion-pairing groups. Obelisc R has many modes of separation which allow it to tune retention. Method is LC/MS compatible and conditions could be replicated to retain many pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%
Buffer Gradient AmAc pH 3.0- 30-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Pesticide, Hydrophobic, Ionizable
Analyzing Compounds 2,4-D
 

Application Analytes:

2,4-Dichlorophenoxyacetic acid