Alachlor Separation on Obelisc and Primesep HPLC Columns

Alachlor is a herbicide which inhibits the gibberellin pathway. Use of alachlor is banned by the European Union and the U.S. EPA. The EURL (European Union Reference Laboratory) includes alachlor in a pesticide analysis of teas and chamomile by liquid and gas chromatography. A single multiresidue method was developed using both Primesep B2 and Obelisc R analyze many pesticides. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations, while Primesep B2 contains embedded basic ion-pairing groups. Method is LC/MS compatible and can be used as a general approach for analyzing alachlor.

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Alachlor
 

Application Analytes:

Alachlor