Amino Sugars Analysis in Ion Exchange and Reverse Mode

Primesep 100 retains an amino sugar by a combination of cation exchange and reversed phase. Columns with reversed-phase functionality alone are unable to retain this amino sugar. The polar interactions of the Primesep 100 column together with the reversed-phase interactions retain the amino sugar and resolve it from its many impurities. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 205 nm.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.5 ml/min
Detection UV 205 nm
 

Description

Class of Compounds  Hydrophilic, Ionizable
Analyzing Compounds Sugars
 

Application Analytes:

Amino Sugar