Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)


Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase.  Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Application Analytes:

Alanine
Amino Acids
Aspartic Acid
Leucine
Methionine
Valine