Complex of Cations on Primesep C is Effected by Organic Modifier

Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. In addition, the elution order can be rearranged by simply changing the organic modifier in the mobile phase from acetonitrile to methanol. The mobile phase mixture of water, acetonitrile (MeCN, ACN) or methanol (MeOH) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).

Condition

Column Primesep C, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeOH, MeCN
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection ELSD 50C
 

Description

Class of Compounds Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sodium, Potassium,  Lithium, Chloride
 

Application Analytes:

Lithium
Potassium
Sodium