Dicamba Analysis on Primesep and Obelisc Mixed-Mode HPLC Columns

 
Dicamba is a widely used herbicide. Dicamba mimics auxin, a plant growth hormone, increasing growth beyond the resource availability of the plant. The EURL (European Union Reference Laboratory) included a QuEChERS (Quick, Easy, Cheap, Rugged, and Safe) method for dicamba as a target pesticide for EUPT-SRM. Obelisc R and Primesep B2 were used to retain dicamba and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing dicamba, other auxin-like phenoxy acids, and dozens of other pesticides.
 

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4.5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4.5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Dicamba
 

Application Analytes:

Dicamba