Dichlorprop (2,4-DP) Analysis on Primesep and Obelisc HPLC Columns

 
Dichlorprop (2,4-DP) is an herbicide similar to 2,4-D. Dichlorprop is used to control weeds and can be found in many weed killers. Only the R-isomer of 2,4-dichloropropanoic acid is used as an herbicide. The EURL (European Union Reference Laboratory) included dichlorprop in a list of acidic pesticides which can be analyzed by a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) single residue method. 2,4-DP was separated by both Obelisc R and Primesep 100. Primesep 100 is a reverse-phase column with embedded acidic ion-pairing groups while Obelisc R is a mixed-mode column with separates using ionic groups and a long hydrophobic chain. Methods are LC/MS compatible and able to be used with many pesticides.
 

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 3 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Dichlorprop (2,4-DP)
 

Application Analytes:

Dichlorprop