Diclobutrazol Separation on Primesep and Obelisc HPLC Columns

Diclobutrazol is a fungicide based on 1,2,4-triazol. It is used in production of coffee and various cereal grains. Primesep 100 Obelisc R were used to separate diclobutrazol. Primesep 100 is a mixed-mode column which uses embedded ion-pairing groups and Obelisc can fine tune separations using both a long hydrophobic chain and ionic groups. Method is LC/MS compatible and can be used for many pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Diclobutrazol
 

Application Analytes:

Diclobutrazol